Welcome to use SupClusterWriter.php

<====Design gRNA super cluster for Cas9 mediated gene editing====>

Select a template
5 prime sticky end*
3 prime sticky end*
Spacer Number [2..25]

*the sticky end is generated by BsaI cleavage

and compatable with gene editing vector.

Please use capital letters for sticky ends

and pay attention to the order for letters of 3 prime sticky end